Septic Shock: Methods and Protocols

Septic Shock: Methods and Protocols

版本:1 edition

作者 :Thomas J. Evans

年份 :2000

编号 :9780585355665, 9780896037304, 0896037304

总页数 :189\189

语言 :English

文件类型 :PDF

大小 :1 MB (1567316 bytes)

简介 :

An interdisciplinary panel of expert investigators describe proven methods for investigating the often deadly mechanisms of septic shock. Drawing on insights from a wide variety of fields-ranging from molecular and cellular biology to the physiological measurement of entire animals-these experienced research workers detail the most important techniques for studying sepsis involving endotoxins, toxins derived from Gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and primary cell cultures. They also treat a number of significant techniques that are not well described in the literature, including the the Limulus endotoxin assay, the bioassay of TNF, the purification of bacterial endotoxin and superantigens, and the culture of primary human cells. Multidisciplinary and state-of-the-art, Septic Shock Methods and Protocols offers both experimental and clinical investigators a gold-standard collection of key methods for illuminating the basic pathophysiological processes that occur following infection.





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